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USA
- USA
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- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 5 - Jul 10
For Your Every Summer RSVP, with Code: SUMMER15
Description
4-HNE (4-Hydroxynonenal) ELISA KitThe 4 HNE (4 Hydroxynonenal) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with 4 HNE. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to 4 HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme
The 4-HNE (4-Hydroxynonenal) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
Shipping Notes
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Exchange/Return Notes
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