Rat EED (Embryonic Ectoderm Development) ELISA Kit
SKU: 87998669253

Rat EED (Embryonic Ectoderm Development) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

Rat EED (Embryonic Ectoderm Development) ELISA KitThe Rat EED (Embryonic Ectoderm Development) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat EED. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EED and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro

The Rat EED (Embryonic Ectoderm Development) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EED. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EED and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EED, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EED. You can calculate the concentration of Rat EED in the samples by comparing the OD of the samples to the standard curve.

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SKU: 87998669253

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4.4 ★★★★★
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M D S
Alexandria, US
★★★★★ 3
Dog loved it, wish it had lasted - better suited for light chewers
Color: Stick
Our puppy was able to pull the rope out of the rubber piece within a day or so. Unfortunately, he then started chewing on the blue rubber piece and had it destroyed in no time. Once the pieces started coming off, we had to throw it away to prevent him from ingesting it.
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Reviewed in the United States on March 20, 2021
B
Verified Purchase
Bernardka Vesel
Lake Worth, US
★★★★★ 5
great for teething puppies
Color: Stick
I have puppies that are 6 weeks old and this toy has been great. They like to gnaw on it as their teeth are erupting. I easy to clean, washer and dryer. I have several other teething toys from same company as I liked this one so much. Would purchase again, highly recommend for teething pups.
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Reviewed in the United States on January 6, 2024
C
Verified Purchase
Chill39
Whiting, US
★★★★★ 5
My English springer spaniels favorite toy!
Color: Stick, Color: Stick
Boomer, my English springer spaniel loves this toy, it is by far his favorite since he was a young puppy. He is three now and although the original toy is still useable it was showing a little wear so I purchased a second one for his third birthday. He was an aggressive chewer when he was younger and I had a great deal of difficulty in finding toys he would not destroy in 30 minutes. I bought the Orka stick to help with teething and it proved to be very durable and became his favorite toy. He loves the new one just as much enthusiasm. boomer doesn't like the rope so I removed it.
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Reviewed in the United States on May 18, 2015
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Verified Purchase
labsrule72
Pawtucket, US
★★★★★ 2
Disappointment
Color: Stick
Disappointment, I have bought these for several of my labs as pups in the past, The center material used to be fleece with knots at ends and difficult To pull through the rubber chew . This toy now has like cotton and polyester strings through the toy tied into knots it easily to chews a part with the dogs, sticks in their teeth and makes a mess, Also easily pulls right through out of the rubber chew toy . After less than one day I ended up throwing away this knotted ball of string and I threaded some fleece strips through the toy and made large nots at the ends, it is now holding up quite well To a pretty aggressive puppy chewer. But I pretty much had to make my own toy
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Reviewed in the United States on September 11, 2022
K
Verified Purchase
Kelly
Houston, US
★★★★★ 4
Good for adult & puppy.
We have a one year old English Mastiff female & a 3 month old English Mastiff male puppy. They both love this toy. I have to say Pet Stages won me over after my mother gave me a few of their toys. I'm not a fan of bright colors, but the dogs just these things. All the toys seem to be designed & tested to keep them interested. My dogs love trying to pull the ropes out, chewing the blue firm but soft enough tube and playing tug-o-war with it.
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Reviewed in the United States on December 8, 2009

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