Protein A magnetic microspheres

Protein A magnetic microspheresProduct Name Protein A magnetic microspheres Product Overview Protein A magnetic beads are a special biotechnology product commonly used in immunoprecipitation (IP) experiments in biomedical research. They are made of superparamagnetic material coated with Protein A, a protein isolated from Staphylococcus aureus that specifically binds the Fc fragment of several mammalian IgGs. Technical Parameter Concentration: 30 mg mL Solvent: water Diameter: ~1um

Product Name

Protein A magnetic microspheres

Product Overview

Protein A magnetic beads are a special biotechnology product commonly used in immunoprecipitation (IP) experiments in biomedical research. They are made of superparamagnetic material coated with Protein A, a protein isolated from Staphylococcus aureus that specifically binds the Fc fragment of several mammalian IgGs.

Technical Parameter

Concentration: 30 mg/mL

Solvent: water

Diameter: ~1um

SPA load : 70-75ug/mg

Surface potential:~ 29 mV

Magnetic content: 35%-45%

 Product Features

High affinity: Protein A magnetic beads are able to bind specifically to Fc fragments of a wide range of mammalian IgGs with high affinity. 

Superparamagnetic: These magnetic beads are made of superparamagnetic material, which can be rapidly magnetised and show magnetic properties under an applied magnetic field, facilitating experimental manipulation and separation. 

Low non-specific binding: Protein A magnetic beads are usually pre-closed to reduce non-specific binding and improve the specificity and reproducibility of experiments. 

High capacity: Protein A magnetic beads have a high binding capacity and can capture a large number of antibodies or antigens.

Application Fields

Application Fields

Immunoprecipitation assays: In cell lysates, the specific binding ability of Protein A magnetic beads allows for the capture of protein complexes bound to their antibodies for downstream analyses such as protein blotting (Western Blot) or mass spectrometry. 

Antibody purification: Due to the high affinity of Protein A for IgG, Protein A magnetic beads can be used to purify specific types of antibodies from mixed samples. 

Affinity Chromatography: In affinity chromatography, Protein A magnetic beads can be used as a solid phase carrier for the separation and purification of target proteins.

Related Information

Sealed, avoid light, and keep at 4¡ãC Expiry date: Six months before unsealing.

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