Rat CK-18/M65 (Cytokeratin 18) ELISA Kit
SKU: 80627133759

Rat CK-18/M65 (Cytokeratin 18) ELISA Kit

Sale price$234.00 Regular price$260.00
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Description

Rat CK-18/M65 (Cytokeratin 18) ELISA KitThe Rat CK 18 M65 (Cytokeratin 18) ELISA Kit measures CK 18 M65 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CK 18 M65 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Rat CK-18/M65 (Cytokeratin 18) ELISA Kit measures CK-18/M65 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CK-18/M65 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CK-18/M65 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CK-18/M65 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 80627133759

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Carlos h
Cuba, US
★★★★★ 5
Good deal. Pretty durable
Size: Large
Dog loves it! Not as crinkly, but still enjoyed even if smashed a bit.
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Reviewed in the United States on February 22, 2026
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JoanieV.
San Leandro, US
★★★★★ 3
Great ball but easily broken
Size: Large
I got this ball for my two dogs, one is a 90 pound yellow lab mix and the other 45 pound black lab mix. So far only my bigger dog has played with it and has also busted it. He was playing with it for probably half an hour just chomping down to make it crinkle and crunch. It's quite the loud ball!! I noticed the sound wasn't as loud so I took a peek and noticed my dog basically crushed the inner ball into a much smaller ball. He's still having a good time with it though. It's only a matter of time though that he's going to chew the outer shell because it doesn't make noise like it did.
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Reviewed in the United States on December 31, 2024
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Mari Sned
Charlottesville, US
★★★★★ 5
Dogs go for this ball every time
Size: Large, Size: Large
My Boston's and Rotti love this ball. I had to get two more. It's great for the Boston's because they can grasp it with the way it's made with the softer rubber. They love the crackle sound. It's just the right size and weight for smaller like the Boston Terrier and Rotti. Easy cleaning, rinses right off.
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Reviewed in the United States on May 13, 2026
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buckzmom
Cuba, US
★★★★★ 5
It's a Hit a Our House!
Size: Large
My strong chewer is still ..... Well, going strong! He loves his new crackle ball. He enjoys the new sound, the bounce back feeling in his jaws when chews. Mom is happy that pup hasn't put a dent in the toy yet! Price was more than reasonable too. We give it 5 Paws Up!
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Reviewed in the United States on May 25, 2026
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Lisa
Belleville, US
★★★★★ 4
Medium size preferred.
Size: Large
I purchased both the large 4" and the medium 3" balls. My 80 lbs German shepherd much prefers the medium size. Show loves to play fetch with it and its lasted well!
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Reviewed in the United States on May 10, 2026

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