Human TSPAN12 (Tetraspanin-12) ELISA Kit
SKU: 66449261766

Human TSPAN12 (Tetraspanin-12) ELISA Kit

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Description

Human TSPAN12 (Tetraspanin-12) ELISA KitThe Human TSPAN12 (Tetraspanin 12) ELISA Kit measures TSPAN12 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TSPAN12 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Human TSPAN12 (Tetraspanin-12) ELISA Kit measures TSPAN12 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TSPAN12 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TSPAN12 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TSPAN12 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 66449261766

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4.1 ★★★★★
Based on 2113 reviews
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phan_graph
Pawtucket, US
★★★★★ 4
Great buy
Color: Blue+Red
My dogs kill every toy. They arrived very agressive chewers. These have lasted way longer than most balls have. Great value
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 24, 2026
M
Verified Purchase
megs2168
Belleville, US
★★★★★ 5
If your dog loves tennis balls, you NEED these
Color: Azure+Orange, Color: Azure+Orange
Raising any puppy is tough. Raising a German Shepherd puppy has often left me questioning my life choices. So I was ecstatic the day that my 60-lb puppy decided that he loves tennis balls and occupied himself for 2 hours without needing constant intervention. Naturally, my immediate response was to make sure we had a constant supply of large tennis balls for dogs available. Now my puppy loves mischief, but he’s not an aggressive chewer. And I was irritated to discover that every brand and size of tennis ball broke within an hour max of play. By simply playing fetch and batting the ball around with his paws, every single tennis ball split into 2 halves. We needed something better! These squeaky balls are exactly that! They come in a 2-pack. I gave my pup the 1st one a week ago and it’s still going strong! We play fetch in the backyard, he brings it into the doggy pool with him, it rolls around in the dirt and can be rinsed off easily. It’s by far his favorite toy, and he takes it everywhere with him. Finally a squeaky ball that can keep up with my dog! These are the perfect size, texture, bounce, squeak, everything. These are the ONLY squeaky balls I will buy from now on. Five stars for sure!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 12, 2026
B
Verified Purchase
Brad
Charlottesville, US
★★★★★ 5
Really are indestructible. Amazing product.
Color: Blue+Red
Wow! Really indestructible. Our large dogs have destroyed other “indestructible” toys in less than an hour and these are amazing. They squeak, which our dogs love (we hate) and they float when they go into our pool. IF they ever do wear out or are lost I will definitely buy again.
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Reviewed in the United States on June 3, 2026
K
Verified Purchase
Kelsey
Draper, US
★★★★★ 5
Durable squeaky ball for chewers
Color: Blue+Red, Color: Blue+Red
These are great! My 54 lb GSP loves them and it’s the only thing she cannot destroy! She’s currently 1 year and we have had the balls for several months. We have this brand and similar ones too. They float, bounce, and squeak. The ridges are good for chewing.
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Reviewed in the United States on May 4, 2026
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Verified Purchase
Stephanie Dutey
Alexandria, US
★★★★★ 5
Finally a Toy My Pitbull Hasn’t Destroyed in 5 Minutes
Color: Blue+Red, Color: Blue+Red
I have a 65 lb pitbull who destroys most toys almost immediately, so I’m always skeptical when products claim to be “indestructible.” Most toys last maybe a few minutes in my house before pieces are everywhere. These have actually held up surprisingly well. The material feels durable and thick enough to handle really aggressive chewing, and so far my dog hasn’t been able to destroy them like he does with most other balls and squeaky toys. That alone makes these worth it to me. I also really like the size. They’re large enough that I don’t have to constantly worry about him accidentally swallowing them or choking while playing, which is a huge plus for bigger dogs. The squeaker inside also keeps him interested longer since he usually gets bored quickly with toys that don’t make noise or bounce around well. If you have a strong chewer or larger dog and you’re tired of replacing destroyed toys constantly, these are definitely worth trying.
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Reviewed in the United States on May 10, 2026

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