Sheep T (Testosterone) ELISA Kit

Sheep T (Testosterone) ELISA KitThis Sheep T (Testosterone) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess

This Sheep T (Testosterone) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2 nm. The concentration of Sheep T in the samples is then determined by comparing the OD of the samples to the standard curve.