Rat Beta-EP (Beta-Endorphin) ELISA Kit

Rat Beta-EP (Beta-Endorphin) ELISA KitThe Rat Beta EP (Beta Endorphin) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat EP. During the reaction, Rat EP in the sample or standard competes with a fixed amount of Rat EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat EP. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase (HRP)

The Rat Beta-EP (Beta-Endorphin) ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat β-EP. During the reaction, Rat β-EP in the sample or standard competes with a fixed amount of Rat β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.