Rat CITH3 (Histone H3 Citrullination) ELISA Kit
SKU: 49870042504

Rat CITH3 (Histone H3 Citrullination) ELISA Kit

Sale price$234.00 Regular price$260.00
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Description

Rat CITH3 (Histone H3 Citrullination) ELISA KitThe Rat CITH3 (Histone H3 Citrullination) ELISA Kit measures CitH3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CitH3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated

The Rat CITH3 (Histone H3 Citrullination) ELISA Kit measures CitH3 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CitH3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CitH3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CitH3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 49870042504

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