Cys-C (Cystatin C) ELISA Kit

Cys-C (Cystatin C) ELISA KitThis Cys C (Cystatin C) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human Cys C. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cys C are added to the micro ELISA plate wells. Human Cys C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys C. Excess

This Cys-C (Cystatin C) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys-C.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Cys-C. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.