Goat CXCL2/GROBETA (C-X-C motif chemokine 2) ELISA Kit

Goat CXCL2/GROBETA (C-X-C motif chemokine 2) ELISA KitThe Goat CXCL2 GROBETA (C X C motif chemokine 2) ELISA Kit measures CXCL2 GROBeta in Goat Serum, Plasma, Cell Culture Supernatant. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CXCL2 GRO antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Goat CXCL2/GROBETA (C-X-C motif chemokine 2) ELISA Kit measures CXCL2/GROBeta in Goat Serum, Plasma, Cell Culture Supernatant. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CXCL2/GROβ antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CXCL2/GROβ bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CXCL2/GROβ in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.